Translational_Unit
RBS+eGFP+T
Part:BBa_K193205:Design
Designed by: Nao Nakatani Group: iGEM09_Tokyo_Tech (2009-10-14)
TmTHP with EGFP for fusion of Anti-Freeze Protein and RBS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1016
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1016
Illegal NheI site found at 1034
Illegal NotI site found at 1046 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1016
Illegal BamHI site found at 551
Illegal XhoI site found at 452
Illegal XhoI site found at 1055 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1016
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1016
Illegal AgeI site found at 761
Illegal AgeI site found at 800
Illegal AgeI site found at 872 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used biobrick standard assembly.
Source
We used B0030 and K193203.